Celastrus Paniculatus Seed Extract Protects Neuron Against MPP+-Induced Toxicity in Parkinson’s Disease Model
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Abstract
Previous studies reported that Celastrus paniculatus had various cellular functions including enhancement of learning and memory. However, there are no reports on neuroprotective effect especially on MPP+-induced toxicity, a model of Parkinson’s disease. The present study aims to demonstrate the effect of C. paniculatus seed extract on inhibition of MPP+-induced SH-SY5Y cells. Cell viability was measured by an MTT assay. Furthermore, the expression of marker proteins of apoptosis, Bcl2 and mTOR, were detected using Western blot. The results showed that 10 and 25 ug/ml C. paniculatus seed extract increased cell viability on incubated with MPP+ for 3 hours. Moreover, C. paniculatus seed extract increased the expression of Bcl2 and decreased the expression of mTOR in compare to control. This study suggested that C. paniculatus seed extract prevented the effect of MPP+-induced SH-SY5Y cells, in vitro cell model of dopaminergic neurons in Parkinson's disease
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