The Study of Morphology and Optical Properties of Wildlife Sanctuary in Thailand with Polarized Light Microscope

Main Article Content

Teeraporn Srinivet
Thiti Mahacharoen

Abstract

The purposes of this research are to study structure and difference of the hairs on Wildlife sanctuary in Thailand using Polarized Light Microscope and to establish the hair characteristics profile of wildlife sanctuary in Thailand. Sample groups of this study consisted of 9 families and 13 species of wildlife. All samples were hairs of the wildlife sanctuary in Thailand which were taken from Dusit Zoo and Safari World Zoo in Bangkok.


Comparison of hair layers which are medulla, cuticle and root hair was performed under 200X and 400X magnification by three techniques; polarized light, crossed polar and medulla index. The accuracy of hair characterization depends on the hair morphology under polarized light, the hair color under crossed polar and the medulla index. The results from the experiments demonstrated that the structure of hairs can be characterized, even in similar species. This is also suggested that, the comparison from only one technique cannot be used to distinguish of different hair type for some species. However, with the comparison under 3 techniques, the morphology, color and medulla index data provided a distinctive characterization. 


This research develops the database for the characteristics of conserved animal hairs in Thailand. It can be an alternative method instead of DNA testing. This will be benefit for the case of destroyed body of the animal with a low cost examination.

Downloads

Download data is not yet available.

Article Details

How to Cite
Srinivet, T., & Mahacharoen, T. (2021). The Study of Morphology and Optical Properties of Wildlife Sanctuary in Thailand with Polarized Light Microscope. Journal of Criminology and Forensic Science, 7(1), 1-11. Retrieved from https://so02.tci-thaijo.org/index.php/forensic/article/view/242905
Section
Research Articles

References

Boonyapat, S. (2010). Estimation of age of human seminal stains by Fourier Transform Infrared Spectroscopy (FTIR). Master of Science Thesis. Silpakorn University, Nakhon Pathom. (In Thai).

Central Information Technology Center. (2020). Criminal offense statistics. Retrieved February 5, 2020. from http://pitc.police.go.th/dirlist/dirlist.php?dir=/crimes

Emilia Lachica. (1997). Study of semen stains by scanning electron microscopy. Influence of their ageing. Journal of Forensic Science International. 91(1), 35-40.

Handbook of Advance Analytical The Center for Scientific and Technological Equipment, Suranaree University of Technology. (2012). Scanning Electron Microscope (SEM). Retrieved April 25, 2019. from http://cste.sut.ac.th/cste/web1/web/link/JR21ntIUgWAa.pdf

Hazelwood RR, Burgess AW. (1995). Practical aspects of rape investigation : A Multidisciplinary Approach. 2nd Edition. New York: CRC Press.

Imsin, A. (2015). Detection of Seminal Stains on ceramic tiles and fabrics by Attenuated Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR). Master of Science Thesis. Silpakorn University, Nakhon Pathom. (In Thai).

Onlamay, S. (2011). PSA Detection of samples with seman under different environment in study. Master of Science Thesis. Silpakorn University, Nakhon Pathom. (In Thai).

Tibkorn, K. (2013). Examination of seminal stain by Attenuated Total Reflection Infrared Spectroscopy. Master of Science Thesis. Silpakorn University, Nakhon Pathom. (In Thai).